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The reservoir solution contained 20% PEG 3350, 0.2M CaOAc.
The drops were formed by mixing 1 µl of protein solution with 1 µl of reservoir solution and equilibrated against 500 µl reservoir solution in each well.
The solution for cryoprotection contained the reservoir solution with the addition of 15% (v/v) glycerol.
Drops were composed of 2 µl reservoir solution and 2 µl protein solution.
Both forms were obtained at 19°C in equal volumes of protein and reservoir solution.
The reservoir solution contained 0.1 M MES and 1.4 M ammonium sulfate pH = 6.0.
Crystallization drops containing 1 µl protein solution (40 mg/ml) and 1 µl reservoir solution.
20% (w/v) PEG 4000 in the reservoir solution was very suitable cryoprotectant for this crystal.
The reservoir solution supplemented with 20% glycerol was used as cryoprotectant.
The volume of the reservoir solution used was 500 550 µl.
The crystal was cryo-protected in the reservoir solution enhanced with 25% ethylene glycol.
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