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The sheared chromatin was submitted to a clarification spin and the supernatant then used for ChIP or reserved as "Input".
One-tenth of starting material was reserved as input DNA control.
50 μl of sonicated lysates prior to antibody addition was reserved as input sample.
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The chromatin solutions were diluted 10-folds using dilution buffer, and 10 µl of each was reserved as total input control.
Dilutions of chromatin preparations were reserved as either input (no antibody) or used for immunoprecipitation with the desired antibody or IgG as a negative control.
Remember to reserve as early as possible.
'Input' of 1% was reserved as control, immunoprecipitation was performed by 4°C overnight incubation with anti-H3K4me3 (Upstate, 04-745), anti-H3K9me3 (Upstate, 17-625; Abcam, 8898), anti-H3K9ac (Upstate, 17-658), anti-H3K27me3 (Upstate, 17-622) anormalmal rabbit IgG respectively.
This character is reserved as the text field delimiter.
Mostly used reserved as calf and weak animals pasture (kallo).
Ten μl of sheared chromatin was reserved to serve as "input DNA".
Following centrifugation (4000 g at 4°C for 5 min), the supernatants were retained and a sample reserved for input DNA.
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