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Amplification was run in a Peltier Thermal Cycler PTC-200-MJ Research programmed with 94 °C for 3 min, 40 cycles of 94 °C for 30 s, 50 °C for 40 s, 72 °C for 1 min, followed by 72 °C for 7 min. The amplified product was sequenced with forward and reverse primers in an ABI sequencer.
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