Briefly 1000 ng of sample DNA was bisulfite treated using the Zymo DNA Methylation Kit (Zymo research, Orange, CA, USA).
DNA was bisulfite-modified using EZ DNA Methylation Kit (ZYMO research, Orange, CA) as described previously [ 18].
Bisulphite treatment of 500 ng template genomic DNA was carried out with the EZ-96 DNA methylation kit (Zymo Research, Orange County, USA) following the manufacturer's protocol.
Bisulphite reactions were performed using the EZ-96 DNA methylation kit (Zymo Research, Orange County, USA) with an input of 1 μg of genomic DNA.
Genomic DNA was converted using the EZ DNA Methylation Gold kit (Zymo Research, Orange, CA, USA).
Bisulphite treatment was performed using the EZ DNA methylation kit (Zymo Research, Orange, CA, USA).
DNA samples were modified using EZ DNA Methylation Golden Kit Zymo Researchh, Orange, CA).
A DNase I (Zymo Research, Orange, California) digestion step was subsequently performed to remove genomic DNA.
DNA samples were bisulphite-modified using the EZ DNA methylation kit (Zymo Research, Orange, CA, USA).
Zymolyase, gel extraction, and DNA clean up kits were obtained from Zymo Research (Orange, CA).
