But employment of this analysis would not necessarily lead in specific circumstances to failures of ratification; the particular facts surrounding the passage of rescission resolutions, for example, might lead Congress to conclude that the requisite "contemporaneous" "expression of the people's will" was not undermined by the action.
The nonrandom requisite for monoallelic expression of a subset of genes has also resulted in the formation of susceptibility loci for neurobehavioral disorders, developmental disorders, and cancer.
More likely, the selection of suitable promoters and plasmids is pre-requisite for regulated expression of heterologous genes.
In the sequence of time, the BMP-6-induced down-regulation of δEF1 transcription occurred at as early as 3 h; whereas up-regulation of E-cadherin was observed only after 12 h of BMP-6 induction, suggesting that BMP-6-induced down-regulation of δEF1 may well be a pre-requisite to allow the expression of E-cadherin.
Although functional expression of the requisite pathway enzymes remains an unsolved challenge, an increasing number of alternative orthologous enzymes and pathways are currently being explored [ 53- 55], which will potentially allow this issue to be overcome in the near future.
Because of the observed early arrest in reprogramming associated with DKC1-depleted MEFs, we next asked whether these cells were able to undergo the mesenchymal-to-epithelial transition (MET), a requisite initiating event prior to expression of SSEA1 antigen (Li et al., 2010; Samavarchi-Tehrani et al., 2010; Golipour et al., 2012; Polo et al., 2012).
So, based on Emx2OS down-regulation in Emx2-null mutants as well as on consequences of OS1-179 overexpressinn in rhombo-spinal precursors, we can speculate now that mutual promotion of sense and antisense transcription at the Emx2 locus may be a crucial pre-requisite for proper activation and expression of Emx2.
One of the many reasons is that we lack the requisite tools for independently controlling the expression of multiple genes in parallel.
However, a recently developed recombinant expression system includes a plasmid for overexpression of the requisite acetylation enzyme NatB and permits bacterial expression of N-terminally acetylated and isotopically enriched aS, needed for NMR studies.
Oliver (Jaeden Lieberher) – who has the requisite saucer eyes, cowlick, scrawny physique and expression of perpetual, cowed innocence – has just moved in, and he's having some problems.
