Hence, efficient hydrolysis of AFEX treated biomass to achieve both high glucose and xylose yields requires supplementing the cellulases with hemicellulases and other accessory enzymes.
To understand precisely the nature of biological systems, therefore, requires supplementing the empirical with the quantifiable, and hence, a synergistic collaboration of experimental and computational methods.
Thus, due to the structural complexity of xylans, efficient hydrolysis of wheat arabinoxylan to achieve high xylose yields by complete xylan monomerization requires supplementing xylanases with α- l-arabinofuranosidases and other accessory enzymes [ 9].
Compared with dominant markers, selection markers based on complementation do not require supplements in the cultivation medium.
Moreover, in vitro differentiation protocols tend to be fairly lengthy and complex; the general biologist's approach tends to require supplementing a number of chemical compounds, biological factors or viral gene vectors, which can lead to high variability between experiments.
The experiments showed that excellent yield of middle distillate products can be obtained from the bio-derived syngas, but it required supplementing the syngas with hydrogen due to low H2/CO ratio.
14. Ignorance and false beliefs are not usually sufficient to make a preference subject to interference, and generally require supplementing by stronger considerations such as age or cognitive disability or harm to others (e.g. one's child) on the basis of one's own ignorance or false beliefs.
Cells were then washed once in sterile dH2O, resuspended to 1.0 OD600 in liquid synthetic minimal medium containing 2% glucose and the required supplements, with or without tetracycline.
The cultures were then grown with or without tetracycline in liquid synthetic minimal medium containing 2% glucose and the required supplements at 30°C and harvested at an optical density at 600 nm (OD600) of 0.5 to 1.0.
