The masking hour is, traditionally, a period of required sequestration — a nighttime ritual designed to rebalance your skin in privacy.
A carrier model requiring sequestration of both water and its driving ligands within intramembranous compartments is inconsistent with an unbroken continuously transmitting pathway, as is required for electrical current or osmotic pressure driven water flows.
Chaperone-mediated autophagy is distinct from other types of autophagy in that it does not require sequestration of the proteins to be degraded (cargo) into any type of vesicles for lysosomal delivery.
What is the function that requires the sequestration of these proteins to filaments?
Although we apparently only detect a small proportion of 'helix-open' NS1 in the cytoplasm relative to nucleus at 8 h post-infection, this may be explained by the fact that NS1-mediated activation of PI3K is a catalytic process, thus requiring few NS1 molecules, whereas CPSF30 inhibition requires stoichiometric sequestration by many NS1 molecules.
Autophagy requires the sequestration of cytoplasmic content or organelles through the formation of double-membrane vesicles, controlled by autophagy-related genes (ATG) and their protein products.
In contrast, microautophagy [ 5], the least studied form of autophagy, requires the sequestration of cytoplasmic substrates within lysosomal membranes per se that are then pinched off to deliver the cargo into the lysosomal lumen.
This process requires the sequestration of the target protein within a double membrane, the autophagosome, the delivery of the autophagosome to the lysosome, fusion with the lysosome and the efficient degradation by lysosomal hydrolases.
"The cuts required by sequestration will be deeply destructive to national security, domestic investments and core government functions," Zients wrote.
He said the cuts required under sequestration would "go right at readiness, right at maintenance, right at training".
