Cas9 is used mostly with CRISPR and represents a DNA endo-nuclease providing a certain advantage for CRISPR compared to other gene editing tools such as ZFN and TALEN, because it does not require repeatedly designing and expressing the Cas9 protein.
This phenomenon, combined with the role for SMRTER in regulating the M/E switch, indicates that SMRTER is required repeatedly at different stages during follicle cell maturation.
In the learning mode, the user is required to repeatedly key in the password for several times (10 20) to stabilize his/her keystroke template.
Unlike French troops, British troops were better trained and were required to repeatedly practice firing with live rounds before encountering combat.
In the finger sequence tapping task which was adopted from Walker and colleagues [21] subjects were required to repeatedly finger-tap with the non-dominant left hand a five-element sequence presented on a computer monitor as fast and accurately as possible on a key board.
A one-off internet survey Y/N v. Longer studies that required you repeatedly provide information over several days/weeks/months Y/N 1.
In the LIGHT IT UP campaign, NIKE ran a contest in which youth were required to repeatedly watch photos and videos of children posing next to the LIGHT IT UP messages [ 10].
Moreover, the WHO cut-off titer was originally based on the adequate immune response levels required when repeatedly monitoring healthy patients who needed a prophylactic preexposure regime on a regular basis, for example, veterinarians (14 ).
Because of the challenges associated with working with the python genome, which has not yet been annotated, students are required to repeatedly validate their results throughout the semester (Table 5).
Athletes engaged in these sports are required to repeatedly produce skilful actions and maximal or near-maximal efforts (eg, accelerations, changes in pace and direction, sprints, jumps and kicks), interspersed with brief recovery intervals (consisting of rest or low-intensity to moderate-intensity activity), over an extended period of time (1 2 h).
