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Interestingly, whereas other aligners appear to use multiple template sequences to align one candidate sequence, this alignment algorithm only requires one reference sequence per candidate sequence and does require explicit knowledge of the 16S rRNA secondary structure.
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SGP2 requires one or more reference genomes to which the target genome (in this case A. mellifera) is compared.
Quality assessment requires one manually generated segmentation (for reference) plus computer-generated segmentations corresponding to different image segmentation algorithms or algorithm parameter settings.
Executing the phylogenetic analysis requires one query sequence and at least two reference strain prototype sequences.
Three references were required – one from the police – before a person was even considered.
Most states require one.
(Some jobs doesn't require one).
I am required by law to include at least one reference to "avocado toast" in any article about millennial housing in Canada.
Its disadvantage is that it requires a significant memory storage since it uses more than one reference curve as shown in [3].
Only one reference is missing.
Just one reference to Google still remains.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com