Whether a device specific square root or power law model is used, it requires off-line or in-line calibration data.
However, unlike CLSM, this multi-color MPM method often requires off-peak excitation of each fluorophore in order to excite all fluorophores in a given sample simultaneously, thereby decreasing the excitation efficiency and making excitation unequal over the given set of fluorophores [ 12].
92 required, off 24 deliveries.
47th over: India 269-5 (Jadeja 16, Dhoni 17) chasing 299 36 required off 24 balls.
It looked anything but that when captain Ravi Bopara was run out with 73 required off 7.1 overs.
Each itinerary is designed to minimise the amount of days required off work while maximising the potential for "outstanding and original experiences".
The America's Cup boats were idle again Thursday, a required off day even though racing has yet to begin in the best-of-three series.
But in the end, with 23 required off Marlon Samuels' final over, England had left themselves with just too much to do.
With 13 required off the final over, victory was still not entirely out of Pakistan's reach.
After some wanton wastage of wickets - Alex Hales and Eoin Morgan the most culpable - 50 were required off five overs.
Then with one required off the last ball, Ansari dived to make his ground and seal a two-wicket win.
