A better understanding of the relative roles of such potential drivers of spatial structure requires further sampling, which for O. marina is ongoing in our research group.
Whether possession of the simA gene is a derived character state, or whether there has been a single or multiple losses within the genus, remains unknown and requires further sampling.
Nevertheless, SBSE, like any other analytical method, has some limitations for extraction of certain compounds and the attainment of such low LOD may require further sample preparation, e.g., pH adjustment, back extraction or derivatisation [34].
Inverse PCR and Southern hybridizations are very laborious techniques that require further sample processing to determine the location of the insertion sequences.
The validation of this hypothesis would require further sampling from within the distributional range of rabai, which is challenging due to many coastal forests having been transformed by humans.
Additional testing for patients suspected of having TB requires further sputum samples.
We suggest that the threat posed by this hazardous organism requires further environmental sampling to assess the distribution and prevalence of this subspecies in Europe.
Proof of this possibility requires further taxonomic sampling of cbf5 genes to find the intron that includes the protein-coding sequence.
Application of these technologies to human fecal samples requires further investigation to determine how these samples reflect metabolism inside the gut and, ultimately, to improve the understanding of the impact of polyphenols on host health [ 12, 31].
Whether the more sophisticated Bayesian model (mtZoa) used for the amino acid analysis has provided a more accurate estimate of interrelationships of Taenia, which requires further testing, additional sampling of nuclear genes, such as complete 18S and complete 28S rDNA [ 61], may be useful in providing 3 independent estimates (morphology, mitochondrial and nuclear DNA) of phylogeny.
