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Accurate assessment of peripheral nerve regeneration requires fixation techniques that preserve tissue in a natural state with minimal artifact.
Certain fluorescence labels may influence biochemical processes in cells or cannot enter live cells to access cytoplasmic molecules (often fluorescence imaging requires fixation of the cells).
Antiserum to RDL does not label cell bodies at all [31], and requires fixation that precludes GFP visualization, and could therefore not be utilized to support the rdl-Gal4 expression.
Studies typically investigate gene expression by using cell lines [15] [17], isolated early-stage limb mesenchyme [18], whole growth plates [19], or laser-capture microdissection, which requires fixation and decalcification protocols, and sectioning to evaluate gene expression in specific regions of the growth plate [20] [23].
Immunostaining of intracellular antigens requires fixation to preserve cellular structure and permeabilization to allow antibody access.
A sample prepared for SEM imaging requires fixation, coating, and dehydration for electron conduction.
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Nine cases required fixation: eight with circular external fixators and one with a monolateral external fixator.
Mann et al. [20] reported that 33% required fixation removal because of symptomatic fixation devices with this method of subtalar joint arthrodesis.
In particular, the Vancouver classification helps distinguish stable from unstable fractures requiring fixation as well as stable from unstable implants requiring revision.
Introduction of large reamers or nail into a narrow canal can result in comminution at fracture site which are usually undisplaced fissures not requiring fixation.
Fractures through the tip of the styloid are likely to be stable [20] (Fig. 4) and do not require fixation as the ulno-radial ligament remains attached to the ulnar head at the base of the styloid.
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