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Very few studies have focused on the bioprocessing aspects of ADI from Pseudomonas sp. As ADI is an intracellular enzyme, its recovery from bacterial cells requires an effective method of cell breakage.
Although this facilitated the primary recovery of protein, the proper folding and resulting bioactivity of the recombinant protein recovered from inclusion bodies requires an effective method to solubilize, refold and purify the protein [10], [24].
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As previously described, the long-term application of any sensing scheme requires an effective encapsulation/attachment method which disallows the undesirable release of key chemistry components.
"Effective regulation requires an effective enforcement program".
With increasing of potential toxicity of engineered nanoparticles (NPs), many in-vivo and in-vitro studies have been conducted and required an effective extraction method of NPs from exposed biological samples (tissues or cells).
Female patients of child-bearing age were required to start an effective method of contraception.
Long-acting reversible contraception ([LARC] i.e. contraceptive implant and intrauterine devices), which do not require daily adherence, offer an effective method to help decrease unintended pregnancies.
Installing a complete MIMIC-II VM on a local computer involves more steps and requires a longer time, but is an effective method when the shared resources for QueryBuilder become the bottleneck in conducting a research study.
This is an effective method that doesn't require you to actively run around squashing spiders with a flashlight.
An effective method that requires less data and expertise would help in the assessment of rehabilitation potential and in the monitoring of rehabilitation activities as complicated theories and excessive data requirements on assemblage information make many current assessment models expensive and limit their wide use.
Manipulation of the beams required a highly effective method for keeping the particles from scattering out of the proper path and hitting the walls of the tube.
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