However, they have some drawbacks, including high background noise, extended time and labor requirements, lack of specificity, lack of sensitivity, and a high entry and operation cost.
Two sets of primers, one newly designed and targeting the nuclear ribosomal DNA internal transcribed spacers (ITS) and the other amplifying a fragment of mitochondrial large subunit ribosomal DNA met the requirements of high specificity and sensitivity, amplifying DNA from a broad range of larger basidiomycetes, with no amplification of plant, bacterial or ascomycete DNA.
The assay was rigorously tested and requirements of sensitivity, specificity, stability and good recovery were fulfilled.
The result (1) was first derived in the context of selectivity of energy requirements and specificity of energy disposal of chemical reactions [ 25, 28].
In molecular imaging, the development of suitable radiolabeled probes requires a rational design based on the structure biological activity biodistribution relationship because the requirement of structural specificity for a radiolabeled probe is rigidity for binding to the respective DNA, mRNA, receptor, transporter and enzyme.
Facing the inadequacy of inductive reasoning, Hempel (1965) added the fifth requirement - requirement of maximal specificity (RMS) for I-S model.
Alternatively, the "provisos" problem implies that satisfying the requirement of maximal specificity may be more demanding than has been generally understood in the past.
On these grounds, we hypothesized that subfamily-C2 establishment and expansion was related to a functional requirement of vomeronasal specificity for V2RC.
Fagen says that the document is "broad and nonspecific in its requirements... the lack of specificity certainly leaves open the opportunity for institutions to water down the purpose of the policy".
Furthermore, compared to ELISA, SISCAPA has the advantage that the specificity of the assay is provided by MS, significantly relaxing specificity requirements of the antibodies used [82].
