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In this section, we aimed to investigate the following question: how well the inference methods meet the requirements of microarray data?
Notably, probes from such batch-wise query meet the requirements of microarray probe design.
Neither KPATH nor Insignia is applicable for designing microarray fingerprints, as the design and specificity requirements of microarray fingerprints are quite different from those of PCR signatures.
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Furthermore, the majority of spleen samples isolated with this kit did not fulfill the quality requirements for microarray or NGS analysis.
All RNA samples fulfilled the requirements for microarray experiments.
Although the number of replicates only met the basic requirements for sample size of microarray studies, the results showed that the immunogenome-focused strategy we used has worked efficiently.
The most well known MIBBI project is still the original MIAME (minimum information about a microarray experiment) checklist (7), a current requirement for the publication of microarray data in many journals.
Ideally, new technologies will reduce sample volume requirements, lessen the amount of time required for microarray analysis (which now averages 24 hours), and reduce microarray costs to the extent that they can be used routinely in the clinic.
Although a number of studies have been published and despite the requirements of some journals to deposit microarray data in public databases like GEO or ArrayExpress, it is still very difficult for researchers to obtain the original data.
A major obstacle for broad application of microarray analysis will be the requirement of fresh tumor tissue.
Rather than using only one framework for all fields of microarray research, these lists satisfy the specific requirements of each field, allowing for arbitrary levels of detail.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com