However, only fluorescence has clinical applicability, while bioluminescence remains a preclinical research tool because of the requirement of expression of luciferase by the genome.
Drawback for the use of this system includes the requirement of expression conditions such as need to follow growth of bacterial culture and add IPTG/NaCl at the proper time.
During interphase, chromosomes and nuclear structures are in constant motion; therefore, correct temporal association is needed to meet the requirements of gene expression.
In an effort to decipher the requirements of p53 expression in vivo, we found the E-box element in the p53 promoter and its binding by c-Myc contributed significantly to high-level expression of p53 reporters in the proliferating compartments.
The VSG pseudogenes could be copies of silent VSG pseudogenes, or partially assembled mosaics, or genes that have degenerated in situ through lack of requirement for expression.
It has been speculated that silent BESs might safely foster the stepwise assembly of mosaics, in contrast to the active BES, from which there is an absolute requirement for expression of an intact VSG [40], [41].
Its other roles include a requirement for expression of the muscle-attachment gene stripe (sr), in the presumptive notum (Ghazi and VijayRaghavan, 2003) and for maintenance of the indirect-flight muscle marker Vg, in AMPs (Sudarsan et al., 2001).
Consistent with the requirement for expression of the sense and antisense RNAs from DualU6GFP, gene silencing via this vector displayed a 24 h delay compared with a synthetic siRNA targeted to the same region of the dEGFP mRNA.
In the 'mutator' mouse ROS production and oxidative damage are generally not significantly increased, suggesting that ROS may not be a necessary requirement for expression of the progeroid phenotype (although see later discussion regarding stem cell aging) [18,59].
"That ran the risk of being incompatible with the convention requirements of freedom of expression".
