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The model is limited in scope because it assumes rather chemically unrealistic requirements for replication and, perhaps, even more importantly, requires "pre-evolution", i.e., the origin of replication, the absolute crucial step in the origin of life, is not addressed.
This putative structure was used as a guide to examine the 3′ requirements for replication in vivo.
Although replication proteins are conserved among eukaryotes, the sequence requirements for replication initiation differ between species.
Remodelling of SVA/LAVA VNTRs at the DNA level, therefore, meets the requirements for replication slippage.
The temperature requirements for replication of these viruses and further transmission are also likely to differ (3 ).
In contrast to nucleic acids, which associate according to the rules of base pair complementarity, the intricacies of protein structure do not – normally – allow for an easy mechanism of replication, which presumably explains the evolution of a coded system for their synthesis (for an interesting discussion of the contrasting molecular requirements for replication and catalysis, see [ 12]).
Similar(54)
Again, this is not a requirement for replication; it is merely one form of a replicator.
The requirement for replication in diverse host species (e.g. mammals and invertebrates) imposes important functional constraints and strong purifying-selection, minimising genetic drift and reducing the rates of nucleotide substitution [26], [77].
If the maintenance of highly diverse swarms equates to increased phenotypic flexibility, then swarm size could be particularly important for arboviruses given their requirement for replication in disparate hosts and tissues.
Our results lead to a minimal fourfold increase in the sample size requirement for replication studies, and also imply that many loci that appeared not to have been replicated may be in fact true positives, but failed to replicate because the replication sample sizes, and thus power, were too low.
The reason for this is not known but may reflect a requirement for S15 in a process required for replication of a damaged DNA template.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com