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An oligonucleotide complementary to the universal primer region, which is present in each probe, can be used as a bridge so that one longer probe can be ligated together from two smaller constructs, and length requirements for probe synthesis are therefore cut in half.
Due to the requirements for probe specificity, probes could not be constructed for all predicted CGIs.
Considering these results we chose a minimum score ratio of 10 and a maximum combined repetitive and low-complexity base content of 5% as the minimum requirements for probe acceptance (configurable).
DNA sequence surrounding a specific polymorphism must meet specific requirements for probe design, including but not limited to: A. genomic screening; the DNA sequence flanking the target SNP must be unique and not have more than 1 genomic alignment hit with 21 or more contiguous bases to ensure annealing specificity, and there is no second SNP nearby.
More than 98.8% of the SNPs were flanked by at least 40 bp on either side and met the requirements for probe design constraints for all genotyping platforms whereas all SNPs met the flanking sequence requirements for an iSelect (Illumina) genotyping assay.
Similar(55)
Polyacrylamide-based glycoconjugates used for a long time for the study of specificity of soluble and membrane-associated lectins fit very well with requirements for probes of lectins.
There are several requirements for a probe to be useful in dissecting the tumor Hsp70 proteome.
This new S-shaped vertical probe satisfies the design requirements for a vertical probe without the guide structure and has the potential for use as a cost-effective guide-free probe card for the wafer-level testing of IC chips.
The minimal requirements of probe number are also presented to provide guidelines for BTT system design.
Ammonia meets the requirements for such a probe.
The minimum requirement for a probe-set to be called 'present' therefore is hybridisation to at least one probe-pair within that probe-set.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com