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However, the precise requirements for origin function either in vivo or in vitro remain unknown.
First, we determine the initiator protein requirements for origin function in vivo in the genetically tractable strain Sulfolobus islandicus (Sis) (Guo et al., 2011; Zhang et al., 2010).
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An important requirement for the origin of life is the concentration of organic compounds to allow interaction with each other and with other chemical species.
Therefore, the widespread production of multiple splice forms could be a main requirement for the origin of functional AS.
By the mid-20th century, scientists had imposed additional requirements for a viable lunar-origin theory.
Our findings reveal that transcription in cis is not required for stage 13 ori62 firing, suggesting the possibility of the requirement of a trans-acting factor for origin firing at DAFC-62D.
A search for empirical rules for superconductivity in materials on X=O, N, C, B… etc. suggests a common origin and requirements for covalency and pairing of radical bonds with focus on the electronegative subspecies.
This unique data resource allows for a rigorous assessment of the requirements for finishing sequence of diverse origins; the current study reveals substantial differences in the effort needed to finish the sequence from different genomic regions and, in particular, from different species.
Importantly, ESC of human and mouse origin differed in their requirements for Cdk2; being critically important for the maintenance of pluripotency in human cells.
There is no requirement for country-of-origin labeling in cut flowers.
Although the times at which origins fire lie on a continuum, early and late origins are distinct in terms of the proteins associated with preorigin complex and the genetic requirements for firing (Santocanale and Diffley 1998).
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