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Other telomeres are also at the periphery but the fraction localized and their requirements for localization vary from telomere to telomere (Hediger et al. 2002).
There are several requirements for localization techniques, including accuracy, robustness, and ease of deployment.
In particular, our results unravel the minimal requirements for localization of the cell division protein FtsZ.
Similar(57)
The basis of this approach is the requirement for localization of complementing β-galactosidase fragments (α – 77 amino acids; ω – 993 amino acids) within the same compartment to achieve enzymatic activity.
Subsequent experiments aim first to establish whether there is a similar requirement for localization of full-length IP3R in the ER, and secondly to define the role of these residues in ER targeting.
Our findings revealed that co-localized mRNAs share global features and have similar cytoplasmic requirements for their localization.
To determine the genetic requirements for the localization of Tudor (Tud) in the nuage, egg chambers mutant for distinct components of the nuage, including Vasa (Vas) [19], Aubergine (Aub) [21], Maelström (Mael) [14], and Krimper (Krimp) [22], were examined.
The different positions of Baz and aPKC are reflected in the distinct genetic requirements for the localization of each protein.
To define more specifically the minimal requirements for ER localization, we used constructs truncated within TMD2 or the succeeding loop.
As highlighted above, in this study we describe mRNA-containing granules in unstressed cells where, even though the mRNA is likely translated, there does not appear to be any specific requirement for the localization of the resulting protein product, nor does the mRNA show a localization pattern indicative of ER, peroxisomal, or mitochondrial localization.
Alternatively, PIK3CA mutations may be weak activators of the PI3K pathway, due to the requirement for plasma localization and/or other activating factors, and require another hit(s) for full activation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com