Sentence examples for requirements for expression from inspiring English sources
(3) Does the island consist of a combination of genes showing different host requirements for expression?
For our analysis we used more stringent requirements for expression (see below) than previous work.
To evaluate whether the structural requirements for expression of shRNA from the CMV promoter, +6 TSS and pA TTS apply not only to luciferase-targeting constructs, two CMV-shRNAs targeting ApoB were designed and named CMV-shApoB1 and CMV-shApoB2, respectively.
The VSG pseudogenes could be copies of silent VSG pseudogenes, or partially assembled mosaics, or genes that have degenerated in situ through lack of requirement for expression.
It has been speculated that silent BESs might safely foster the stepwise assembly of mosaics, in contrast to the active BES, from which there is an absolute requirement for expression of an intact VSG [40], [41].
Here, we demonstrate that the minimal requirement for expression (transcription and translation) is the two IR-directed sense-oriented TYLCV genes V1 and V2.
Its other roles include a requirement for expression of the muscle-attachment gene stripe (sr), in the presumptive notum (Ghazi and VijayRaghavan, 2003) and for maintenance of the indirect-flight muscle marker Vg, in AMPs (Sudarsan et al., 2001).
In the 'mutator' mouse ROS production and oxidative damage are generally not significantly increased, suggesting that ROS may not be a necessary requirement for expression of the progeroid phenotype (although see later discussion regarding stem cell aging) [18,59].
Consistent with the requirement for expression of the sense and antisense RNAs from DualU6GFP, gene silencing via this vector displayed a 24 h delay compared with a synthetic siRNA targeted to the same region of the dEGFP mRNA.
Unlike sea urchins, both insects and vertebrates have multiple Runx genes [ 2], which may reflect the greater regulatory network complexity in these organisms (that is, the Runx paralogs may reflect a requirement for expression variants as opposed to functionally distinct proteins).
We sought therefore to generate variegated expression with simple transgenes and then to identify molecular requirements for variegated expression.
