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H. influenzae is confirmed by using Gram stain and factor X and V requirements (Differentiation Disks; Difco Laboratories, Detroit, MI, USA).
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Table 3 Selection of quality requirements Requirement Differentiation: 100% compliance found?
At confluency, experiments were initiated to determine hormonal requirements for differentiation.
The criteria that the expert panel used to select quality requirements were: Differentiation between EDs: if all EDs in the sample already met the quality requirement, it was not included in the QRF.
This specific hypo-responsiveness makes sense considering that CD4 coreceptor upregulation in transgenic mouse thymus rescued T cells with weakly interacting TCR provoked a skewing of the T-cell populations, and CD4 coreceptor lineage-committed thymocytes have stringent signaling requirements for differentiation.
Is zinc depletion a hallmark of pluripotency, or is zinc increase a requirement of differentiation?
Although our data do not allow to infer if calpain-like molecules are actually involved in T. cruzi metacyclogeneis, the transcriptome of several T. cruzi indicated that TcCALPx11 is up-regulated in epimastigotes under nutritional stress [39], a requirement for differentiation to metacyclic trypomastigotes [4].
We find that loss of Cdh1 is a limiting requirement for differentiation of pluripotent cells, and that experimental suppression of Cdh1 activity rescues the BMP-imposed block to differentiation.
The TFs Runx2 and Osx are considered as 'master switches' and absolute requirements for osteoblast differentiation [21] - while Runx2 is essential for MSC differentiation, Osx acting 'downstream' of Runx2 controls osteoblastic fate determination [48,49].
In addition, some cells differentiated into heterocysts without cell division after nitrogen step-down, suggesting that cell division in the mother cells is not an essential requirement for heterocyst differentiation.
We also observed cells that differentiated into heterocysts without cell division after nitrogen step-down, suggesting that cell division among mother cells is not an essential requirement for heterocyst differentiation.
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