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Employing quantifiable reporter genes, such as GFP present in the AZE-GFP virus and RT43.2GFP vector, replaces the requirement to detect viral protein expression in productively infected cells [4], [5] or the use of selectable markers such as the G418r gene employed in the previously reported gammaretroviral vector studies [6], [7].
The only requirement to detect full-length Ulp1 at the septin ring is the C580S mutation and functional Kap121.
The larger sample size of 600 patients in each group allowed adequate exposure data to be collected for the regulatory requirement to detect rare cardiovascular events across the entire linagliptin program.
The complexity of the DDR is in part a function of the requirement to detect and respond to a wide variety of DNA damage events and to regulate the numerous potential outcomes of the genetic insult.
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Important issues for these experiments were the high accuracy and position requirements necessary to detect a possible failure wave or failure kinetics in SiC-ceramics as might be evidenced by a change in the slope of the penetration velocity impact velocity curve.
Thus, the value of exploring requirements to detect errors and optimize requirements prior to system implementation cannot be overstated.
Sample size requirements to detect uncommon events such as deaths and disabilities together with challenges of household surveys of women in the Islamic cultural context indicated that a population-based school survey of youth in generally large families was the most feasible study design.
We also characterize the implications of a multisite versus single site study design for statistical power, including a figure that shows sample size requirements to detect activation in two key nodes of the working memory circuitry given observed differences in reliability of measurement between single and multisite designs.
While this was a single-site study, the sample size exceeded requirements to detect significant differences in compliance over time.
Sample size requirements to detect a certain difference in PRO could be larger if different administration formats are used because of greater standard deviations and because effect estimates are likely to be attenuated by additional (non-differential) measurement error [ 9, 10].
The problems with measurement imprecision in studies of gene-lifestyle interaction are eloquently outlined and discussed by Wong et al. (19), where the authors provided estimates of sample-size requirements to detect gene-lifestyle interactions in the presence of varying degrees of environmental exposure assessment and phenotyping measurement error.
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