These results indicate a high level of requirement for components of the Oct4 network in early mouse development.
Accordingly, we explored the activation of autophagy by obatoclax, the requirement for components of the autophagy machinery and the implications for cell fate.
Cells that are not growing are expected to have a relatively low requirement for components of macromolecular synthesis; on the other hand, protein synthesis is required during differentiation, since new proteins are needed.
Standards use the concept of Safety Integrity Levels (SILs) to drive the assignment of system safety requirements to components of a system under design.
Non-functional requirements of component based systems are important as their functional requirements, therefore they must be considered in components assembly.
A compositional grading of PEEK and PTFE is implemented in this study to create a bulk composite with the functional requirements of component strength, stiffness and wear resistance while providing solid lubrication at the sliding interface.
Of interest, however, our work and that of other laboratories has suggested that the requirements for components of this canonical TCR signaling pathway may not be absolute, and that observed requirements may reflect, at least in part, events that limit the ability of alternative molecular mechanisms to support pre-TCR/TCR-driven T cell development.
In any event, our findings further reinforce the requirement of NMD components, including UPF2, for active proliferation in mammalian cells although our data from the fetal liver shows that there are important cell type- and/or developmental-specific differences.
Finally, the continual requirement of the components of the RAS-directed ZNF304 pathway to maintain silencing has therapeutic implications.
One plausible hypothesis, supported by global gene expression analysis, is the requirement of additional components of the basal transcription machinery in order to accommodate a more elaborate gene regulatory framework in these organisms that is linked to development in metazoans [ 56].
The character of the host rock is the key factor determining the design and specific requirements of individual components of such a facility.
