However, the requirement for complementarity of only seven or eight nucleotides could result in hundreds of possible targets (Lim et al, 2005).
Each CRISPR spacer, however, is in principle constrained to target a specific phage because of the requirement for complementarity to the entire RNA sequence (though see below on the possibility of CRISPR seed sequence).
To eliminate the potential misleading influence of these sequences, which may be under selection due to the requirement for complementarity between miRNAs and their targets, on the similarity test, each foldback arm with hits (E<0.05) in the FASTA search was analyzed independently with and without the miRNA or miRNA-complementary sequences.
Binding of the 5' end of the guide strand to the target mRNA appears to be critical, with an almost absolute requirement for complementarity at the so-called "seed site" from positions 2 7 (measuring from the 5' end of the guide strand) [ 9- 11].
The requirement of base-pair complementarity at the nick junction has been explored for development of ligase-based technologies for mutation detection.
Both the rapid evolution of viruses and the necessity of precise gene regulation could have worked as selective pressures towards the modern miRNA pathway since the requirement for a high degree of complementarity between plant miRNAs and their targets can act as a stabilizer, preventing sequence drift even over long periods of evolutionary time [ 43].
In general, Bohr considered the demands of complementarity in quantum mechanics to be logically on a par with the requirements of relativity in the theory of relativity.
Heisenberg's uncertainty principle and Bohr's notion of "complementarity" are superbly dramatized.
"This kind of complementarity is fine in the short term," the senior French official said.
The automotive industry likes the idea of complementarity because it keeps people buying cars – albeit for use over shorter distances.
