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Tissue-engineered vascular grafts require long fabrication times, in part due to the requirement of cells from a variety of cell sources to produce a robust, load-bearing extracellular matrix.
The different effects of nutrient addition to the two media probably reflect the higher maintenance requirement of cells fermenting SH.
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Our mutational analysis suggests that at least GTP binding by Gpn2 and Gpn3 is an absolute requirement of cell viability; thus all three GPN proteins require GTP.
To meet the requirement of cell biology, the next generation micro-nanorobot should have small, automatic, high integration and applicable features in multiscale.
However, the structure of traditional acellular nerve scaffold is dense; the interval porosity and void diameter are too small to meet the requirement of cell seeding, which limits the application.
Notably, more than 10 HSP/DNAJs co-chaperones might indicate a strong requirement of cell repair as well as important TF translocators [126].
However, the data regarding the requirement of cell-cell contact is less clear.
Although the role of PGE2 in NK cell-MSC has been reported, the requirement of cell-cell contact for PGE2 induced immunosuppression remained unexplained.
However, despite the fact that we demonstrated the requirement of cell interactions, NC cells remain a mesenchymal population.
10.7554/eLife.04177.024 Figure 4. Requirement of cell-to-cell contact between NK cells and DCs for the enhancement of NK cell-mediated killing activity.
Furthermore, the requirement of cell surface receptors for viral transmission implied, in agreement with ultra-structural data, a junctional border between the nanotube and the connected T cell.
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