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Although many of the mechanisms proposed for probiotic activity probably require growth and metabolism at the physiological site of action, confirmation of the requirement for viability through design of clinical studies with a non-viable control would clarify this issue.
Surprisingly, studies of Sno mutants in both flies and mice have proven enigmatic in revealing developmental roles for Sno proteins, particularly with regard to any requirement for viability.
Rapid growth thus may not be a biological sensible requirement for viability in many wild organisms.
Yet, as it is a strict maternal-effect lethal gene, its requirement for viability is apparent only in the embryo.
While we show that one functional copy of USP4 or USP15 is a minimum requirement for viability (Table 3), the observed departure from Mendelian ratios may arise from a functional deficiency in oocytes haploinsufficient for USP15.
In contrast, strains with the psd1Δ mutation alone or in combination with psd2Δ are not viable on lactate as a carbon source unless rescued by addition of 5 mM Etn to the medium as a minimum requirement for viability.
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Richard Wagoner acknowledged the company has a lot of work to do to satisfy the government's requirements for viability, but said it is doable.
An overarching challenge in associated research and development is to devise material/system architectures which satisfy all requirements for viability in a particular application area, such as light-duty vehicular transportation.
These interactions may indicate a requirement for the transport of specific cargo(s) in the glc7-E101Q muthat tharearequiredred for viability.
City Hall said the new approach would "offer developers a new quicker route through the planning process, removing the requirement for protracted viability negotiations if they meet the minimum 35% affordable housing".
Also, for certain essential genes we suspect that there may be a low degree of gene expression requirement for bacterial viability.
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