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This increases selectivity and sensitivity in complex matrices (especially when trace analysis is required), which was a significant requirement for this study.
There is no ethical requirement for this study, as it utilises published data.
An additional requirement for this study is that the CRPS is located in one extremity only.
Formal ethical approval was not sought as there was no requirement for this study, due to the observational nature of the research.
The informed consent requirement for this study was exempted by the ethics committee because researchers only accessed retrospectively a deidentified database for analysis purposes.
The sample size requirement for this study was calculated considering clinically relevant a difference of 45% in the proportion of patients responsive to budesonide treatment between the groups with positive and negative saline test result.
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As first step, the participants defined the major objectives of preliminary dose-range finding/MTD toxicity studies in non-rodents, defined the principles and requirements for this study type and agreed on a proposal for an optimised study design, based on collective experience of conducting such studies in industry, involving an evaluation of 100 individual study data sets.
There were no ethical requirements for this study.
The present survey had a straightforward sampling plan (a nationally representative sample in terms of age and gender), which served to control costs; a more-complex quota plan (e.g., introducing a requirement for a minimum number of respondents that do not hold a driving license) would have had corresponding impacts on resource requirements for this study.
As a pre-requirement for this study, we first tested the expression of adiponectin receptors (AdipoR) 1 and 2 in the cell lines used.
Weaned pups can be captured, handled, transported and penned at this age, all crucial requirements for this study.
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