In noncycling cells, nonhomologous end-joining (NHEJ) allows the direct reconnection of severed DNA termini without the requirement for a template.
However, many methods, for example those which utilize PCR to create small amplicons are not constrained by a requirement for large template molecules and can in principle be supported using DNA amplified from sorted chromosomes.
FFPE tumor DNA presents a dual challenge for mutation testing, namely requirements for low template input quantities combined with template damage from the fixation and embedding process that resist amplification by PCR.
R. prowazekii has been shown to utilize regulated promoters and to organize genes into operons [4], [5], [6], [7], [8], [9], and rickettsial RNA polymerase was shown to exhibit properties that distinguish it from the Escherichia coli polymerase, such as the requirement for a supercoiled template for promoter binding, [10], [11].
Some other appealing factors of the Bioplex platform include its requirement for minimal DNA template, true liquid phase kinetics, short running time, ease of data analysis and affordability.
Although conventional sequencing produces accurate data, the requirement for knowledge of template sequences and the inability to quickly process multiple targets hinder its practical application in epidemiologic and diagnostic investigations.
Rad51 plays a role in the strand break repair through homologous recombination while Ku70 and Ku80 are involved in non-homologous end joining repair pathway without the requirement for a homologous template [ 31].
Based on these conditions, we again investigated the two requirements for all templates to determine the dynamic range of our digital counting system (Figs 4a,b, and S5).
Until recently, one of the major bottlenecks of shotgun metagenomics has been the requirement for large initial DNA template quantities during library preparation.
The requirement for transfection of DNA templates for intermediate and late gene expression indicated a defect in viral genome replication in VACV-infected S2 cells, which was confirmed by direct analysis.
