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This is likely due to an absolute requirement for stabilizing the nucleotide base by providing either electrostatic or hydrophobic interactions (or both).
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To probe the requirements for stabilizing the hexamer, we systematically varied the polarity and steric bulk of one of the Phe residues in the supercore of the hexamer.
Furthermore, vinculin is required for stabilizing adhesions under force.
The lack of a requirement for primer stabilizing interactions on such substrates is probably due to the established position of the primer strand through interactions with the template strand.
Modulation Transfer Spectroscopy for Stabilizing Lasers" Leo Hollberg, B. Dahmani.
Evidence for stabilizing selection in a eukaryotic cis-regulatory element.
An additional adjusting term is employed to stabilize the system and relax the requirement for an initial stabilizing control.
Thus, the requirement for oligomerization to stabilize SpoIIE in the forespore can be uncoupled from the requirement for oligomerization to activate σF.
The gene-3-protein is essential for the phage infectivity, and we exploited this requirement for a proteolytic selection of stabilized protein variants from phage libraries.
Consistent with a functional crosstalk between the Ddc2– Mec1 and 9 1 1 complexes during checkpoint signaling, there is a reported requirement for the 9 1 1 complex to stabilize Ddc2 foci in irradiated G1 cells (Barlow et al. 2008).
Going forward, as the bar for the plan requirements stabilizes, it is likely that the Agencies' will want to supplement the plan reviews with testing.
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