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Some entire mt genome sequences are available for some species in GenBank, and hence they requirement for sequencing is reduced.
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In light of the conservation of protein structure and degeneracy of sequence, SCOPE was developed to enable the "shuffling" of distantly related genes with no requirement for sequence identity.
Considering the high diversity found in forest trees [ 20- 25], the requirement for sequence conservation in regions surrounding a SNP may be too conservative.
Because our analysis depended on sequences obtained in routine genotypic assays, the requirement for sequences from at least two time points per patient may have resulted in biased sampling of the study population.
It is noteworthy that the sequences labeled TR2 in the teleost lineage are two residues shorter than teleost and tetrapod TR1 and tetrapod TR2, which are the same length (Fig. 4C), supporting a difference in the requirement for sequence constancy between the two lineages, but it is unclear how this relates to the potential gene duplication events.
aThe procedures and estimated time requirements describe the process for sequencing a BAC clone with a size of 100 kb.
Although the separate design of RNAi sequence and barcode sequence can decouple the requirement for RNAi sequence and array hybridization sequence, it also doubles the effort to design and synthesize the library, and the barcodes are still short oligos detected by single probes which inevitably will result in a higher noise level than multiple probe detections of mRNAs.
Previously, anchor identification among multiple genomes has been achieved using pairwise alignment tools like BLASTZ through progressive alignment tools like TBA, but the computational requirements for sequence comparisons of multiple genomes quickly becomes a limiting factor as the number and scale of genomes grows.
This discordance could reflect requirements for sequences outside of the seed region of the two miRNAs for binding to their targets or interaction with RNA binding proteins.
The non-requirement for sequence similarity data allows metrics to be calculated for genomes that are the sole examples within a clade, however superimposing an orthology analysis onto a metrics-based characterisation should provide information regarding the lability of orthologous systems, and how their gene/domain organisation has evolved.
Secondly, many chimeric sequences may have been removed because of the strict requirement for proper sequence tag and primer sequences on the left and right ends, and because of a requirement for long overlapping segments of a defined length.
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