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Results indicate a requirement for sampling over at least 5 consecutive days, even when using total faecal collection.
Our results also emphasize the requirement for sampling far more than one night of sleep to adequately quantify bout duration distributions.
The requirement for sampling is a further barrier to research.
It loosens the requirement for sampling frequency and can obtain high-range resolution by mixing the echo with the radar reference signal.
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As a result, differential analysis has a higher requirement for samples.
This illustrates that our BYEC classifier converged very fast and the low requirement for sample size.
Experimental evaluations were conducted to demonstrate the robustness, low requirement for samples, and adaptiveness of the proposed method.
This microplate-based measurement could not only improve the detection efficiency of the nanosensor with high-throughput characteristics, but also reduce requirement for sample volumes because of microcavities on plates.
Third, and most importantly, we combine five selected features and prove that our method has high accuracy, is adaptive, and has a low requirement for samples in our experiments.
Another disadvantage to this approach is the requirement for sample homogenization.
The requirement for sample pre-amplification for this technology contrasts with standard two-step RT-qPCR approach, therefore the impact of this methodology was also evaluated.
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