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Such a letter is a requirement for modification applications under the government's Making Home Affordable program.
There is no requirement for modification of the internal protein sequence by removing, for example, undesired cysteines.
Nor is there any requirement for modification of the protein at the chemical level by misincorporation of unnatural amino acids or by further modification of the expressed protein.
The above data suggest that if N-terminal residues are physiologically important and potential targets for low-level Rfa2 phosphorylation, there might be a requirement for modification of multiple residues (i.e., a critical mass of phosphorylation) to affect RFA's role in the DNA-damage response.
Similar(56)
Today, such muteins can be routinely expressed in Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris, but the functional requirement for modifications such as disulfide bridges or delicate N- and O-glycosylation present at, e.g., the linker domains of the cellulase proteins render most of these expression hosts inappropriate.
ITC is the straightest path to complete the thermodynamic characterization of protein interaction without the requirement for chemical modification or labeling [157].
Recent research efforts have focused on incorporation of these catalysts into solid, electroactive supports, which can be challenging due to the requirement for synthetic modification to bpy ligands.
The drug interactions were classified into three groups, minor (55.29%), moderate (23.08%) and major (21.63%) according to the severity of and requirement for drug modification.
From this point of view, Lim et al. reported MAS5 superiority and the requirement for GCRMA modification when using human GeneChip data.
MMP activity is itself regulated by a variety of mechanisms, including a requirement for enzyme modification to elicit maximal enzymatic activity and the activity of specific TIMPs [ 25].
Further experiments are required to identify the downstream effector that links SPINK1 to invasion and survival and explains this differential requirement for protease modification.
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