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The activated N protein then stimulated a polyphosphoinositide-specific phospholipase C by reducing the Ca2+ requirement for expression of this activity from superphysiological to normal intracellular concentrations.
The VSG pseudogenes could be copies of silent VSG pseudogenes, or partially assembled mosaics, or genes that have degenerated in situ through lack of requirement for expression.
It has been speculated that silent BESs might safely foster the stepwise assembly of mosaics, in contrast to the active BES, from which there is an absolute requirement for expression of an intact VSG [40], [41].
Here, we demonstrate that the minimal requirement for expression (transcription and translation) is the two IR-directed sense-oriented TYLCV genes V1 and V2.
In the 'mutator' mouse ROS production and oxidative damage are generally not significantly increased, suggesting that ROS may not be a necessary requirement for expression of the progeroid phenotype (although see later discussion regarding stem cell aging) [18,59].
Its other roles include a requirement for expression of the muscle-attachment gene stripe (sr), in the presumptive notum (Ghazi and VijayRaghavan, 2003) and for maintenance of the indirect-flight muscle marker Vg, in AMPs (Sudarsan et al., 2001).
Similar(52)
Analysis of 127 genes that had been trapped by random (nontargeted) gene trapping with the same vector shows that virtually all are expressed in ES cells above this threshold, suggesting that targeted and random trapping share similar requirements for expression levels.
For our analysis we used more stringent requirements for expression (see below) than previous work.
(3) Does the island consist of a combination of genes showing different host requirements for expression?
To evaluate whether the structural requirements for expression of shRNA from the CMV promoter, +6 TSS and pA TTS apply not only to luciferase-targeting constructs, two CMV-shRNAs targeting ApoB were designed and named CMV-shApoB1 and CMV-shApoB2, respectively.
Furthermore, breeding of Snap23fl/wt mice failed to generate homozygous Snap23fl/fl pups, a finding that is consistent with the failure of the floxed allele to express and the requirement for Snap23 expression for embryo viability.
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