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We have previously reported that primary Schwann cells expressing the SV40 Large T antigen (LT) are not fully transformed in that they maintain a strict requirement for attachment, requiring a further genetic change, such as oncogenic Ras, to gain anchorage-independence.
In this system, the non-transformed parental 3T3 cell line exhibited an almost complete requirement for attachment, as only a few small colonies were observed which did not show continuous growth when cultures were extended up to two months (Figure 4 A C).
Previous characterisation of the oncogenic pathways required to transform these cells showed that, as for other cell types, expression of SV40 Large T antigen (LT), which inactivates the p53 and Rb pathways, permits the cells to proliferate mitogen-independently but they retain a strict requirement for attachment and exhibit contact inhibition of proliferation.
Anchorage dependence: the requirement for attachment in order for cells to proliferate.
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"What are the three requirements for attachment?" she asked.
Requirements for attachment to the polymer include a compound with an amine for linking to the polymer.
Since proteins isolated from the cement cone are rich in glycine and this structure may have a role in attachment and since the various species of ticks have different requirements for attachment, herein we perform initial tests of the hypothesis that there are not only anatomical, but also chemical differences between the cement cones produced by these three species of ticks.
To this end we chose to study NIH3T3 cells, since these cells retain numerous features of untransformed cells including cell-cell contact inhibition or the requirement for substrate attachment for productive growth and proliferation.
Among such potential barriers are digestive proteases (trypsins and chymotrypsins), the peritrophic matrix and the requirement for parasite attachment to the midgut epithelia to prevent excretion of parasites with remnants of the digested blood.
Additive manufacture (AM) enables the fabrication of patient-specific medical implants that conform to specific surgical geometries, mimic the mechanical properties of natural bone, and enable enhanced cellular interaction, including tailored microtextures that are tuned to the requirements for cellular attachment and growth.
To determine the structural requirements for the attachment of EPG to Glu362, several HA-tagged deletion mutants of T. brucei eEF1A were designed (Fig. 5B) and transfected into T. brucei procyclic forms.
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