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We relaxed the requirement for alignment to both strands for the first and last 100 bp of the assembly, and we trimmed any remaining terminal ambiguities.
Even with the imminent availability of sequenced scaffolds from high throughput methods there remains a pressing requirement for alignment with sequenced BACs and unequivocal assignment and orientation to respective chromosomes.
Similar(58)
Finally, requirements for alignment of components and steering of the electron beam along the beamline are presented.
A complete substitution of Mg2+ by Mn2+ reduces the activity of archaeal RNAP to approximately 40% (data not shown); the reduced activity is probably caused by the slightly larger Mn2+ ions causing suboptimal spacing [ 47], and Mn2+-catalyzed reactions being more promiscuous in their requirements for alignment of the reactive groups [ 48].
Each 36 bp read was first shortened to 32 bp (a requirement for the alignment tool) and aligned to the grape reference genome [24] using Illumina's ELAND alignment tool.
The width of each corridor was heuristically set to the square root of the number of gaps that would be allowed given the offset-predicted alignment length and input percent identity requirement for the alignment.
It is worth mentioning that the proposed low complexity signal processing complements the work in [4] because the requirement for block alignment is loosened.
Lack of deformation was a necessary requirement for precise alignment of depths of recordings and cortical layers as well as for reliable recordings.
The configuration of two pressure nodes near the sidewalls of the channel narrower than a half acoustic wavelength reduces the requirement for the alignment of IDTs with the channel.
All tests shown indicate that both the finite-difference and the finite-element versions of the scheme should substantially alleviate the requirement for field-alignment of the coordinates over the realistic range of χ∥/χ⊥ in toroidal magnetic confinement devices.
The EVD model eliminates the requirement for additional alignments that are used to generate the background distribution, and thus speeds up the computation for a pair-wise comparison of ligand-binding sites by at least 100-fold.
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