The aqueous solutions obtained were filtered, concentrated in a water bath under vacuum, frozen, lyophilized (Eyela, model FDU-2000, Jandn), and stored at −20°C until required (yield; 12.3%).
Briefly, IgG ELISAs were performed using FMP2.1 as the capture antigen, in serial 2-fold dilution, and the titer was defined as the serum dilution required to yield and optical density of 1.0 in our assay.
After obtaining the required cRNA yield and incorporation rate of fluorescent dye cyanine 3-CTP, a hybridization step was carried out simultaneously for all three biological repeats.
The practical development of pDNA vaccines requires high yield and productivity of supercoiled plasmid DNA (sc-pDNA).
Developing yeasts for massive oil production requires increasing yield and modifying the profiles of the fatty acids contained in the oil to satisfy specific technical requirements.
Compared with particle swarm optimization (PSO), micro-genetic algorithm (micro-GA), and genetic algorithm (GA), the proposed method is found to significantly reduce the number of structural analyses required and yield the better optimum frame designs.
After optimizing the methodology, the peptides were obtained in good purity (no purification was required) and yield.
Therefore, when calculating the sample size using functional outcomes, a smaller sample size would be required and yield greater experimental power.
We describe and apply lacZ transcription reporter plasmids designed for both biochemical analyses requiring high DNA yield and physiological studies requiring low gene dosage.
This "Newly Revised High Throughput EST" allowed the analysis of a higher number of substances, while less manual work was required, and yielded data comparable to those obtained with the conventional EST.
