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Where CFSE labeling was required whole splenocytes were incubated in PBS at 5 × 10'7 cells/ml with 5 mM CFSE (Molecular Probes, Eugene OR) for 10 min at 37°.
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"These would require whole new infrastructures at a cost of trillions of dollars," he said.
Her blessing, and her curse, was a facility for quotable aphorisms that, like Nietzsche's, require whole books to reveal their unconventional meaning.
Other than that, no state has laws that require whole, unprocessed or fresh foods to be available outside of school lunches for high school students.
Some readers seem to feel that restaurants bully diners — with dress codes, say, or with policies that require whole parties to be present before they're seated — and that restaurants don't show enough flexibility or appreciation.
However, event-related paradigms can be exploratory, therefore requiring whole brain scans and so repetition times (TR) of several seconds.
Presence of noise seems to be crucial for descriptiveness of histograms presented in [5], as they require whole data of good quality for proper representation of the object.
Usually, hairs left behind at a crime scene are associated with DNA testing, but such a procedure requires whole, intact hairs and is often time-consuming.
While cell therapies hold great potential for treating many conditions, their utility for treating patients that require whole organ replacement is unclear.
Purpose: To treat children with retinoblastoma, who require whole eye radiotherapy, with a specially designed 125I applicator that irradiates the eye while sparing the surrounding tissues.
These assays are laborious requiring whole animal sectioning to ascertain tissue localization.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com