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Since the Dna2 helicase was not required, we asked what helicase might participate to convert the DSB into a substrate recognized by Dna2, which recognizes only single-stranded termini.
When required, we asked probing questions to ensure that all issues were addressed and understood correctly.
To determine the number of resources required, we asked three physicians to create a treatment profile for each scenario, which were later validated from existing care protocols [ 71- 78].
Similar(57)
Since phosphorylation of EIIANtr was required for suppression, we asked whether the other components of the phosphoryl relay were also required by determining whether overexpression of ptsN could still suppress the essentiality of rpoE in strains lacking ptsP or ptsO.
Since J147 increases NGF and NGF is a trophic factor required for cholinergic neurons, we asked if J147 is effective in an assay dependent upon cholinergic transmission.
As phosphorylation of Akt1 is required for cell survival, we asked if the phenotype of LY was driven by selective death of ADE precursor cells.
We asked whether glyoxalases are required to survive desiccation stress.
Next, we asked whether Pcdhgs are required for formation of SAC SAC synapses.
Next, we asked if Sert is required for guidance at the midline.
Next, we asked whether ATP6AP2 is required for cognitive processes in Drosophila.
Using this technique, we asked whether NFATc2 was required for VEGF-A-stimulated tubulogenesis (Fig. 6D).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com