In identifying differentially expressed genes, we required at least a twofold change in expression and a P value of less than 0.05.
Power analysis indicates that four to seven tissue fragments are required to detect a twofold change in gene expression with a 25% sampling error (see Table 2).
Using the edgeR package (Robinson et al., 2010), we identified significant changes in RNA abundances between control and Rfx2 knockdowns, requiring greater than twofold abundance changes and a false discovery rate (FDR) less than 5%.
Human enzyme activity is precipitated by antibody at concentrations about twofold greater than required for comparable complexation of rat sulfite oxidase.
Thus, ligamers designed to loop out various lengths of a template RNA can be used to condense by more than twofold the information required to assess RNA connectivity 244 nt of the target RNA was condensed to a 104 nt DNA.
The formation of VDAC1 dimers and higher molecular mass complexes was enhanced over twofold upon exposure to Antp-LP4, with the concentration of EGS required to attain 50% of the maximal level of dimers being decreased twofold.
Tetraploidization is, according to the model, unlikely to happen by a change of gamete patterns alone, but requires a more than twofold increase in survival or reproductive output of both male and female LLRR.
These twofold implications require that full transparency and accountability is enforced within research institutions and research funding agencies to ensure that potential conflict of interest do not arise or are effectively managed.
