Sentence examples for required two rounds of from inspiring English sources

Bailout bitterness is a factor: BofA required two rounds of taxpayer assistance during the financial crisis, for a total of $45 billion — money the bank repaid in December 2009, just before Moynihan took over.

The generation of the tetracycline-inducible reporter plasmids required two rounds of stable transfection.

Anergy induction in naïve allogeneic T cells by both lowGM-DC and highGM/4-DC required two rounds of stimulation by immature DC.

Although integration of the selectable marker per se was enough to disrupt the FXN gene, the need to produce knockout of both FXN alleles still required two rounds of transfection with CRISPR-I4 and the targeting construct because simultaneous homozygous FXN knockout is a rare event.

It required three rounds of voting at the federation's general assembly, but Paris prevailed convincingly over three larger, significantly more expensive alternative venues in the suburbs: Gonesse to the north, Versailles to the west and Marne-la-Vallée to the east.

Approval of all HCA items for cognitive debriefing required three rounds of back translation review (step 7).

She may require two rounds of voting to win the presidency, but Lula's extraordinary popularity and the desire for continuity should ensure that the top job stays in the hands of the Workers Partyy.

The advantage of adapter-ligation PCR is that only a single round of PCR is required, whereas spPCR often requires two rounds of nested PCR.

The nested pathway analysis approach has the disadvantage of requiring two rounds of processing.

The strategies require two rounds of PCR amplification using two flanking primers and one internal mutagenic primer.

Teams and individuals differ in their behavior in particular when the game has an intermediate complexity (of requiring two rounds of iteration).