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When the children were given the toy, they often performed only the actions required to activate it.
One likely hypothesis might be that the later the gene is the more cofactors are required to activate it.
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In HsHDAC510, the C-terminal catalytic domain lacks an active pocket required to activate its enzymatic activity.
Although PI3K is not required to activate RAC, it is still essential for fibroblast migration in gradients of LPA, indicating that PIP3-regulated pathways distinct from RAC activation contribute to GPCR-driven chemotaxis.
It should be underlined, nevertheless, that while rapamycin seems to be quite toxic during development and in neonates, it seems to be well tolerated in older animals where, moreover, only one to three injections are required to activate DiCre, and thus it could be used, given appropriate controls, as an inducer of DiCre in adult animals.
Tyrosine phosphorylation is required to activate Syk kinase activity and function.
It has been postulated that sustained JNK activation is required to activate death-promoting downstream targets such as Bim and Bak [ 41].
In case such a substituent is stable to conditions required to activate other types of leaving groups, it can be used as glycosyl acceptor as well.
We then tested whether Notch activity was required to activate Spi/Egfr signaling.
However, the nuclease activities are not required to activate ATM after DNA damage or telomere deprotection.
It is likely that due to prior induced Col2a1-driven recombination, constant Col2a1 activity is not required to activate the Tet-on system in our transgenic mouse.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com