Sentence examples for required supplements from inspiring English sources

Cells were then washed once in sterile dH2O, resuspended to 1.0 OD600 in liquid synthetic minimal medium containing 2% glucose and the required supplements, with or without tetracycline.

The cultures were then grown with or without tetracycline in liquid synthetic minimal medium containing 2% glucose and the required supplements at 30°C and the morphology of cells were examined with a ZEISS AXioskop2 microscope at 400× agnification.

The cultures were then grown with or without tetracycline in liquid synthetic minimal medium containing 2% glucose and the required supplements at 30°C and harvested at an optical density at 600 nm (OD600) of 0.5 to 1.0.

When required, supplements such as leucine, uracil, lysine and histidine were added to a final concentration of 50 mg/l, while the antibiotic nourseotricine sulphate (cloNAT, WERNER BioAgents, Germany) was added to a final concentration of 100 mg/l.

Thus, the use of polyamines to reduce the Mg2+ requirement for group II intron function provides a potential solution to the problem of double injection into D. melanogaster embryos, enabling the introduction of RNPs and required supplements in a single injection that gives chromosomal gene targeting.

Matrigel (BD Bioscience) or extracellular matrix (ECM, Sigma, St . Louis MI) was added to 24-well plates in the volume of 300 µl and was allowed to solidify at 37 °C for 30 min. The equal volume of medium was added and was incubated for another 30 min. BM EPC (2×106) were then seeded and were cultured with required supplements.

The experiments showed that excellent yield of middle distillate products can be obtained from the bio-derived syngas, but it required supplementing the syngas with hydrogen due to low H2/CO ratio.

Compared with dominant markers, selection markers based on complementation do not require supplements in the cultivation medium.

Of the 37 patients (13.2 %) requiring supplements at discharge (group 2), 14 (5.0 %) required Ca tablets alone, and 23 (8.2 %) required both Ca and calcitriol supplements.

The FDA monitors the safety of dietary supplements, but it does not require supplements to adhere to certain guidelines or standards before it is available on the market.

Vegans and vegetarians with additional restrictions on certain types of foods may have additional needs requiring supplements or special dietary changes during pregnancy.