Sentence examples for required supplemented from inspiring English sources

> -wrap-foot> aHofologue of Streptomyces protein (Kaneda et al. 2001) A single colony of the different B. subtilis strains was transferred from a plate to 10 ml Luria-Bertani (LB) medium (if required supplemented with 2 μg/ml erythromycin and 100 μM IPTG) and grown over night (37°C; 300 rpm).

Prmt4+/+ and Prmt4−/− MEFs (mouse embryonic fibroblasts) and HeLa cells were cultured as described previously [ 13, 14] and, where required, supplemented with 400 nM TSA (Calbiochem)/5 mM nicotinamide or 20 μM AdOX (adenosine dialdehyde) for 24 h prior to harvest and/or 500 μM sodium arsenite for 30 min.

The experiments showed that excellent yield of middle distillate products can be obtained from the bio-derived syngas, but it required supplementing the syngas with hydrogen due to low H2/CO ratio.

M. genavense culture is fastidious, requiring supplemented media.

As reported recently, iron-containing proteins require supplemented iron sources which were not added in this case [ 21].

Moreover, in vitro differentiation protocols tend to be fairly lengthy and complex; the general biologist's approach tends to require supplementing a number of chemical compounds, biological factors or viral gene vectors, which can lead to high variability between experiments.

Hence, efficient hydrolysis of AFEX treated biomass to achieve both high glucose and xylose yields requires supplementing the cellulases with hemicellulases and other accessory enzymes.

To understand precisely the nature of biological systems, therefore, requires supplementing the empirical with the quantifiable, and hence, a synergistic collaboration of experimental and computational methods.

Matching the rapid growth in the availability of large data sets with knowledge about how they were generated will require supplementing big data with 'small data', for example from clinicians, patients and qualitative researchers.

Thus, due to the structural complexity of xylans, efficient hydrolysis of wheat arabinoxylan to achieve high xylose yields by complete xylan monomerization requires supplementing xylanases with α- l-arabinofuranosidases and other accessory enzymes [ 9].

Cells were then washed once in sterile dH2O, resuspended to 1.0 OD600 in liquid synthetic minimal medium containing 2% glucose and the required supplements, with or without tetracycline.