Sentence examples for required supplement from inspiring English sources

Although the university vowed to retain its essays in a required supplement, the demise of the "Uncommon Application" sparked a student protest.

B- The required supplement was aliquoted for 400 mg/l final, then purified water was added to make a supplement solution at 40 mg/ml; the appearing suspension was opaque-yellowish.

The experiments showed that excellent yield of middle distillate products can be obtained from the bio-derived syngas, but it required supplementing the syngas with hydrogen due to low H2/CO ratio.

Cells were then washed once in sterile dH2O, resuspended to 1.0 OD600 in liquid synthetic minimal medium containing 2% glucose and the required supplements, with or without tetracycline.

The cultures were then grown with or without tetracycline in liquid synthetic minimal medium containing 2% glucose and the required supplements at 30°C and harvested at an optical density at 600 nm (OD600) of 0.5 to 1.0.

When required, supplements such as leucine, uracil, lysine and histidine were added to a final concentration of 50 mg/l, while the antibiotic nourseotricine sulphate (cloNAT, WERNER BioAgents, Germany) was added to a final concentration of 100 mg/l.

The cultures were then grown with or without tetracycline in liquid synthetic minimal medium containing 2% glucose and the required supplements at 30°C and the morphology of cells were examined with a ZEISS AXioskop2 microscope at 400× agnification.

Thus, the use of polyamines to reduce the Mg2+ requirement for group II intron function provides a potential solution to the problem of double injection into D. melanogaster embryos, enabling the introduction of RNPs and required supplements in a single injection that gives chromosomal gene targeting.

Matrigel (BD Bioscience) or extracellular matrix (ECM, Sigma, St . Louis MI) was added to 24-well plates in the volume of 300 µl and was allowed to solidify at 37 °C for 30 min. The equal volume of medium was added and was incubated for another 30 min. BM EPC (2×106) were then seeded and were cultured with required supplements.

A new federal law requires supplement manufacturers to report serious adverse effects to the Food and Drug Administration, but it depends on consumers to call in reactions.

But Senator Richard Durbin, Democrat of Illinois who sponsored the law that requires supplement producers to file such reports, said the agency needed to both enforce it and make the filings available to consumers.