Sentence examples for required sequencing depth from inspiring English sources

The qualified DNA library was then loaded on Hiseq2000 for high-throughput sequencing, to meet required sequencing depth with 90-bp paired-end reads.

Compared to RNA-Seq, the reduction in complexity of tag-based approaches significantly decreases the required sequencing depth for a good coverage of both the pro- and eukaryotic transcriptomes [ 10- 12], which is a prerequisite for profiling of low abundant pathogen-derived transcripts.

Accurate quantification of total cfDNA will aid future clinical implementation of such approaches through quality assurance of technical performance (e.g. ensuring input quantity is sufficient to achieve the required sequencing depth).

Similarly, when evaluating a given sample it is important to know whether the required sequencing depth has been reached, or if more sequencing is necessary to detect a given transcript, isoform, or fusion gene.

To achieve the required sequencing depth at a reasonable cost, we used target enrichment to sequence SNP markers and developed a statistical framework based on model selection to estimate intervals of mapped QTL peak locations.

Sequencing of the converted DNA immediately reveals the degree of methylation at any genomic cytosine by counting the number of observed cytosines vs. thymines; however complete methylome profiling using this method requires sequencing depths far beyond what is feasible today on the scale of larger patient cohorts.

Chromosome isolation methods do not require sequencing coverage to the depth needed to resolve possibly heterozygous positions [ 10].

While increasing pool sizes above 100 might increase mapping resolution, this would also require more sequencing depth.

These simulations indicate that a high (>90%) chance of detecting QTL with at least 5% effect requires 5000× sequencing depth for a trait with heritability of 0.4−0.7.

The underrepresentation of bacterial RNA in preparations of total RNA from infected host cells requires high sequencing depth to gain statistical significance and adequate pathogen coverage, leading to increased costs.

Messenger RNA-Seq (mRNA-Seq) is based on poly(A) capture, thereby requiring less sequencing depth, and will reflect more faithfully the coding transcriptome, although it will not account for coding transcripts that are not poly-adenylated [ 80, 81].