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We were unable to demonstrate a strong interaction of the truncated proteins with the required sequence present in the msn lamellocyte enhancer (data not shown), suggesting an additional protein(s) may be needed to facilitate or stabilize this protein-DNA interaction.
Any biologically required sequence experiences evolutionary pressure (in this case purifying selection) resulting in a narrowing of the range of allowable sequence mutations.
In fact, the number of orthologs did not increase when decreasing the minimum required sequence identity levels to as low as 50%% in any gene family (data not shown).
In order to confidently phase these regions, we required sequence data for both parents and three children (including the NCO event recipient); such data were available for two pedigrees.
Additional file 1, Table S4 gives the numbers of singletons obtained when varying the minimum required sequence identity and coverage of the blastp hits in the range from 70-10070-100%
Exonerate (Slater and Birney 2005) was used with the parameters "exhaustive" (which turns all heuristics off and allows for the most sensitive search), "percent10" (which sets the required sequence identity threshold for putative hits to 10%), and "est2genome" (which models cDNA to genome alignments and allows for alignments to be interrupted by nonconserved regions such as introns).
Similar(53)
The approach ideally required sequencing 30 billions units of DNA -- 10 times that in a single genome.
But testing each patient required sequencing billions of units of DNA and cost $5,000.
The interconnection of the function blocks according to the required sequences is implemented using a scheduler concept.
Out of 3,264 total sequencing attempts (large exons required sequencing several overlapping PCR products), 3,076 successful sequences were achieved.
> The required sequences for an MRI of the abdomen are almost similar to the MRI protocol of the liver.
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CEO of Professional Science Editing for Scientists @ prosciediting.com