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We noted that unlike the introduction of H3K9me1 which absolutely required replication, the introduction of H3K9me2 and H3K9me3 appeared to occur even when replication was substantially blocked by inhibitor [ 8].
However, we note that the method is designed for data from the discovery stage alone when a follow-up sample is generally not available and the required replication sample size is to be determined based on the genetic effect size estimated from the discovery stage.
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On the other hand, since Real-Time PCR is very sensitive and can detect low viral load and does not required replication-competent viruses, we could not exclude that some detections could be due to carriage or co-carriage in the case of co-infections.
Our findings require replication in independent samples.
This exploratory result requires replication.
Viruses are molecular machines sustained through a life cycle that requires replication within host cells.
It has limited external validity and requires replication in other contexts.
Admittedly, they are preliminary and require replication in a bigger sample for confirmation.
Possible gene-environment interactions arising from these studies also require replication.
Although chances of overfitting are reduced using LOOCV and boosting models,11 current results are preliminary and require replication by independent research groups, in larger samples.
Given this and the heterogeneity of bipolar disorders, these findings should be interpreted cautiously and require replication.
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CEO of Professional Science Editing for Scientists @ prosciediting.com