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If required, remove an aliquot of the suspension for protein content analysis.
If required, remove a suitable aliquot for protein content determination and transfer the liquid into a clean plain glass wide-neck vial (sample vial) while carefully measuring and recording the volume.
Do not use a water bath to defrost a sample; the sample can be warmed by using your hand to the point at which a small amount of ice remains, thus indicating that the sample temperature is still close to 0 ° C. If required, remove a suitable aliquot for protein content determination (10 20 μl) and store it separately.
When required, remove from the freezer and cook.
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Bringing it into the museum for a recent show required removing its wheels, bumper and gasoline (flammable liquids are not allowed in the museum).
About half came from cadavers; the other samples were taken from patients undergoing surgery that required removing some brain tissue.
He tried all the wine preservation devices but ultimately, he says, "they all required removing the cork, and once that's removed the wine is exposed to oxidation.
In some cases this required removing non-homologous genes and gene fragments from poorly annotated genomes.
In the SITVIT domain, this required removing clades that do not commonly infect human beings (e.g., PINI1 and PINI2).
To construct the single-stranded LPPs (ssLPPs) required removing one strand from a double-stranded precursor molecule using exonuclease digestion.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com