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We also required reads to match the exon sequence on each side of the junction exactly.
Finally, we required reads from both the plus- and minus-strands be detected to consider a candidate site to be a bona fide footprint.
With high multiplexing and a low number of required reads for a sufficient sequencing depth, miRNA-seq becomes a popular platform for miRNA profiling with tens or even hundreds of samples, which makes the web based applications or applications that process one sample at time impractical.
To accomplish this, all reads that failed to align to the reference genome were aligned (using Bowtie) to a database of all RefSeq annotated exon-exon boundaries generated such that each junction required reads to overlap each exon by at least 3 nucleotides.
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It is required reading.
All of them required reading.
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It had been required reading during rehearsals.
His catalog notes are required reading.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com